Recovery of endophytic Beauveria bassiana on a culture medium based on cetyltrimethylammonium bromide

ABSTRACT

Selective media (potato dextrose agar plus yeast extract, different concentrations of cetyltrimethylammonium bromide (CTAB) and different antibacterials) were evaluated for the isolation of endophytic Beauveria cf. bassiana from dry bean (Phaseolus vulgaris) plants. CTAB amounts of 0.15 and 0.3 g/L plus either dihydrostreptomycin, oxytetracycline or doxycycline resulted in the lowest numbers of common saprophytic fungi and the highest proportional recovery of Beauveria colonies from internal plant tissues.

KEYWORDS:

• Beauveria bassiana
• culture medium
• endophytic
• entomopathogenic fungi

Additional information

Funding

This project was supported No. 1330-3611-2243 “Endophytic colonization of Beauveria sp. and Metarhizium brunneum on citrus and their action against Diaphorina citri”, Universidad Autonoma Agraria Antonio Narro, Saltillo, Coahuila, Mexico.