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ABSTRACT
Anthracnose leaf spot and ripe rot disease, caused by Colletotrichum gloeosporioides in India, is of economic importance. The objective of the investigation was to isolate and characterise bacterial antagonists from the grape berry surface. Nine bacteria out of the 46 that were isolated significantly inhibited C. gloeosporioides in a dual culture assay. Six of these nine microorganisms demonstrated pathogen inhibition by producing extracellular volatile and non-volatile compounds. Bacteria SB5, SB13, and SB15 were chosen for the polyhouse experiment because they demonstrated the best disease control in a detached leaf assay. At 1 × 106 CFU/ml of bacterial concentration, both curative and preventive trials showed significant disease inhibition. Based on morphological and molecular characterisation the bacteria were identified as Bacillus species. All three strains could be effectively used for the biological control of C. gloeosporioides.
Acknowledgements
The authors would like to thank the Director, ICAR- National Research Centre for Grapes, the Director and Head of school, MIT School of Bioengineering Sciences and Research for their valuable suggestions and support during the study.
Disclosure statement
No potential conflict of interest was reported by the author(s).
Data availability
The sequence of 16s rRNA region of Bacillus aerius strain Cg1, Bacillus velezensis strain Cg2 and Bacillus subtilis strain Cg3 had been deposited in GenBank with accession number MZ677267, MZ677265, and MZ677235, respectively.