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Environmental Technology Volume 32, 2011 - Issue 14
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Original Articles

A microfluidic device for bacteria detection in aqueous samples

Ashish K. Jha Department of Chemical Engineering, University of Rhode Island, Kingston, RI, 02881, USA; Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, 94720, USA
,
Anubhav Tripathi Division of Engineering, Brown University, Providence, RI, 02906, USA
&
Arijit Bose Department of Chemical Engineering, University of Rhode Island, Kingston, RI, 02881, USACorrespondence[email protected]
Pages 1661-1667 | Received 09 May 2010, Accepted 09 Dec 2010, Published online: 30 Jun 2011
 
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Abstract

We demonstrate the use of a microfluidic device that accurately monitors bacteria levels in water. Fluorescent antibody labelled E.coli K12 bacteria in aqueous samples are flowed through the device, and spikes in the emission signal correspond to the presence of bacteria. Target bacteria could also be detected while suspended in a suspension of very low antibody concentration, suggesting that washing of bacteria could be avoided to make the detection automated and more rapid. Use of a microfluidic device will eventually enable quick on‐site detection of bacteria using small quantities of sample.

Acknowledgements

This work was supported by grants from the NSF (CBET 0730392, 0854115) and by the URI Partnership for Ocean Instrumentation.

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