Variation of dissolved oxygen and redox potential and their correlation with microbial population along a novel horizontal subsurface flow wetland

Abstract

Aims: This study was conducted to evaluate the performance of a novel horizontal subsurface flow wetland (HSFW) in naturally improving the dissolved oxygen (DO) and the impact on redox condition, microbial activity and the nitrogen removal in the HSFW bed.

Materials and methods: The HSFW, equipped with cascaded natural aeration ditches (NADs), was the second stage of a hybrid constructed wetland (CW) after vertical-baffled flow wetland beds. The performances of the HSFW for organics and nitrogen removal in a full-scale hybrid CW system treating municipal wastewater for more than three years have been analysed. The spatial distributions of the oxidation-reduction potential (ORP), DO, microbial population density and specific oxygen uptake rate were determined, and their correlations were analysed in one selected section of the HSFW bed.

Results: A 7-m-long shallow NAD increased the DO concentration from 0.28 mg O₂ l⁻¹ to 3.80 mg O₂ l⁻¹ and the ORP from+37.3 mV to+247.7 mV, creating an aerobic zone with a hydraulic retention time (HRT) of 0.5 h and an anoxic zone of another 0.5 h in series in the subsequent wetland bed. For the whole HSFW with three NADs, the macro aerobic and anoxic environment with a total HRT of 3 h can be created.

Conclusions: The unique DO distribution in HSFW may contribute to an optimum environment for partial nitrification and anammox, and obtain a high performance for nitrogen removal. Correlation analysis showed that the microbial activity in the HSFW relied obviously on the redox condition.

Keywords:

• dissolved oxygen
• horizontal subsurface flow constructed wetland
• microbial activity
• municipal wastewater treatment
• redox potential

Acknowledgement

The authors are grateful to Dr K. Kujawa-Roeleveld, Sub-department of Environmental Technology, Wageningen University, the Netherlands, for her first review of this manuscript. Funding for this study was provided by the Ministry of Science and Technology of China with the National Science and Technology Major Project: Water Pollution Control and Management (2009ZX07315).