Inhibitory effects of quinoid redox mediators on a denitrifying culture

ABSTRACT

Denitrification with p-cresol as the electron source was studied in the presence of three quinones at different molar concentrations (0–2 mM): menadione (MEN), alizarine (ALZ) and anthraquinone-2,6-disulfonate (AQDS). Results showed that denitrifying yields were not altered (0.9), but the substrates’ consumption efficiency was mainly affected when adding MEN. In the presence of ALZ and MEN, specific consumption rates decreased 40% for p-cresol and 90% for nitrate. The sludge previously exposed to quinones was submitted to recovering denitrifying studies using acetate and p-cresol. After exposing to AQDS and ALZ, the metabolic activity of denitrifying sludge was completely recovered. The previous exposition to any MEN concentration resulted in a very low metabolic recuperation. The results show that MEN and ALZ have a marked inhibitory effect on substrates’ consumption and the AQDS does not affect at all. The evidence suggests that MEN modifies the transport system of substrates and ALZ forms a complex with molybdenum. A model based on oxido-reduction potentials of the enzymes involved points out that the influence of quinones tested appears to be more associated with quinone moiety structural properties and hydrophobicity.

KEYWORDS:

• Denitrification
• p-cresol
• menadione
• alizarine
• AQDS

Disclosure statement

No potential conflict of interest was reported by the authors.

ORCID

Arturo Cadena Ramírez http://orcid.org/0000-0003-2813-8186

Additional information

Funding

This work was supported by the Consejo Nacional de Ciencia y Tecnologia (CONACyT) under Grant [SEP-2003-CO2-43144/A-1].