ABSTRACT
The significant amounts of agriculture residues such as bean straw (BS) in rural areas, advises its valorisation for energy recovery. The feasibility of using BS for biogas production through anaerobic digestion was assessed. Prior to this, a fungal pre-treatment to hydrolyse BS with Pleutorus ostreatus was studied at 30°C and 100 rpm in orbital incubators with 1, 10 and 30 mg fungus/g straw for 14, 21 and 28 days. Then, anaerobic digestion experiments were performed in batch with cow manure (CM) as co-substrate and pre-treated BS at ratios (g/g total solids) of 1/2, 1/3, 1/5 and 0/1. Maximum lignin (18%) and hemicellulose (44%) degradation occurred at 30 mg fungus/g straw and 28 days, along with the highest total methane yield (38 mL CH4/g VS loaded). The total amount of methane decreased when increasing CM in the experiments (701.4–191.5 mL CH4), suggesting inhibition owed to a component of CM. Self-sustained biogas production of BS occurred due to the presence of bacteria (i.e. Bacilli and Bacteroidia) and archea (i.e. Methanobacteria and Methanomicrobia). However, the usage of a full-active inoculum should be studied for higher biogas production rates.
GRAPHICAL ABSTRACT
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Acknowledgements
This work was financially supported by the ‘Instituto Politecnico Nacional’ research grant IPN 20150837. The technical support and facilities provided by the staff of the Bioengineering lab at the UPIIZ-IPN are acknowledged. The co-authors P. Becerra Castañeda and José de Jesús Montoya Rosales were financially supported by the scholarship IPN BEIFI 2014 and 2015.
Disclosure statement
No potential conflict of interest was reported by the authors.