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Abstract
The development of aerobic granules was studied in four column‐type sequential aerobic sludge blanket reactors fed with different substrate concentrations ranging from 500 to 3000 mg l−1 COD. Results showed that aerobic granules successfully formed in all reactors fed with different substrate concentrations, indicating that the formation of aerobic granules is independent of the substrate concentration. The granule size, roundness, compactness, physical strength, as well as cell surface hydrophobicity and cell polysaccharides contents of the cultivated aerobic granules were investigated. It was shown that aerobic granules formed with different substrate concentrations had similar roundness and compactness. However, the size of aerobic granules slightly increased with an increase in substrate concentration, while granule strength decreased with substrate concentration. It was found that there was a significant increase in cell surface hydrophobicity and cell polysaccharides of the aerobic granules compared to that of seed sludge. The high cell surface hydrophobicity and high cell polysaccharides contents were believed to play an important role in the formation of aerobic granules. However, substrate concentration seems not to be a governing factor for the formation of aerobic granules. The results of this study would be useful for developing aerobic granules‐based bioreactor and for better understanding of the mechanism of aerobic granulation. It was also clearly demonstrated that aerobic granules‐based bioreactor would have great potential in the treatment of high‐strength wastewater.