Kinetic parameters and inhibition response of ammonia‐ and nitrite‐oxidizing bacteria in membrane bioreactors and conventional activated sludge processes

Abstract

Ammonium and nitrite oxidizing biomasses (AOB and NOB) were investigated in parallel pilot plants: a membrane bioreactor (MBR) and a conventional activated sludge process (CASP) fed with domestic wastewater. The kinetics of AOB and NOB were monitored through titrimetric tests. The maximum specific growth rate of the AOB (µ_max,AOB) was affected by the solids’ retention time (SRT) maintained during the start up: by varying the start up SRT from 20 d to 8 d, µ_max,AOB in the CASP varied from 0.45 d⁻¹±0.04 to 0.72 d⁻¹± 0.2 respectively; the mean value of µ_max,AOB in the MBR samples (always maintained at SRT = 20 d) was in the range 0.45–0.49 d⁻¹. The endogenous decay coefficients of the NOB and AOB and the maximum specific growth rates of the NOB were similar in both MBR and CASP. Inhibition tests with different concentrations of allylthiourea (ATU) were carried out on samples from both activated sludge systems: the MBR sludge exhibited higher sensitivity to a low ATU concentration; however, the maximum nitrification activity recovered more rapidly than the CASP sludge.

Keywords:

• ammonia oxidizing bacteria (AOB)
• nitrite oxidizing bacteria (NOB)
• membrane bioreactor (MBR)
• nitrification kinetics
• nitrifiers inhibition