Effect of river water exposition on adhesion and invasion abilities of Salmonella Oranienburg and Saintpaul

Abstract

This study was performed to evaluate in vitro the adherence and invasiveness capacity of Salmonella Oranienburg and Saintpaul (isolated from river water) exposed to laboratory and river water growth conditions and inoculated into epithelial HEp-2 cell. Results showed that Salmonella Oranienburg and Salmonella Saintpaul showed lower ability to adhere and invade epithelial HEp-2 cells under both growth conditions as compared to Salmonella Typhimurium reference strain. S. Oranienburg adhesion capacity was not affected by the growth conditions, while S. Saintpaul exposed to river water significantly (p < 0.05) decreased its adhesion capacity by 75.7 %. On the contrary, S. Oranienburg exposed to river water reduced its invasion efficiency by 80 %, whereas S. Saintpaul showed no differences between growth conditions. In conclusion, this study suggests that the exposure to non-host conditions, such as river water, adversely affects the adhesion and invasiveness of Salmonella serotypes differently, impacting on their ability to re-enter a new host.

Keywords:

• Salmonella
• pathogenicity
• epithelial cell
• environment
• growth condition

Acknowledgments

Authors thank to Consejo Nacional de Ciencia y Tecnologia (CONACyT) and Convocatoria de Investigacion Cientifica Basica SEP-CONACyT 2010 for its financial support. The authors also thank personnel from the National Food Safety Laboratory Research (LANIIA-CIAD), MC. Areli Burgueño Roman, Dr. Luis Alfonso Amarillas Bueno and Q.F.B. Celida Isabel Martínez Rodríguez for technical assistance. This study was funded by the Consejo Nacional de Ciencia y Tecnologia (CONACYT) Grant ID 164,601 (to CC). The funders had no role in study design, data collection and interpretation, or on the decision to submit the work for publication.