Diazinon toxicity in hepatic and spleen mononuclear cells is associated to early induction of oxidative stress

ABSTRACT

Diazinon is an organophosphorus pesticide, which may have potential toxic effects on the liver and immune system; however, the underlying mechanisms remain mostly unidentified. This work is aimed at evaluating the oxidative stress and cell cycle alterations elicited by low-dose diazinon in a rat liver cell line (BRL-3A) and spleen mononuclear cells (SMC) from Wistar rats. Diazinon (10–50 μM) caused early reactive oxygen species (ROS) generation (from 4 h) as well as increased O₂^•- level (from 0.5 h), which led to subsequent lipid peroxidation at 24 h, in BRL-3A cells. In SMC, diazinon (20 μM) produced similar increases in ROS levels, at 4 and 24 h, with the highest O₂^•- level being found at 4 h. Low-dose diazinon induced G1-phase arrest and cell death in hepatic cells and SMC. Therefore, diazinon could affect the liver and the immunological system through the premature oxidative stress induction.

Abbreviations: O₂^•-: superoxide anion radical; ROS: reactive oxygen species; SMC: spleen mononuclear cells; TBARS: thiobarbituric acid reactive substances

KEYWORDS:

• Diazinon
• Hepatotoxicity
• immunotoxicity
• ROS

Acknowledgments

This study was supported by grants from Programa Iberoamericano de Ciencia y Tecnología para el Desarrollo (CYTED IBEROAMERICA Acción109AC0371), Secretaría de Ciencia y Tecnología-Universidad Nacional de Córdoba (33620180101257CB); Ministerio de Ciencia y Tecnología de la provincia de Córdoba (PID 2018; GRFT 2019; PE 2019 THECA FREE), Agencia Nacional de Promoción Científica y Tecnológica (PICT 2015-2810; PICT 2019-04329). VSM and MGT are investigators from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET-Argentina). GATI is a doctoral student from the Programa de Doctorado en Ciencias Biomédicas, Universidad Nacional Autónoma de México (UNAM) and has received CONACyT fellowship (287281).

The content of this work is the exclusive responsibility of their authors and does not necessarily represent the official views of the organisations that funded this research.

Disclosure statement

The authors report no conflict of interest.

Additional information

Funding

This work was supported by the CYTED Ciencia y Tecnología para el Desarrollo [Acción109AC0371]; Ministerio de Ciencia y Tecnología de la provincia de Córdoba [PID 2018; GRFT 2019; PE 2019 THECA FREE], Agencia Nacional de Promoción Científica yTecnológica [PICT 2015-2810; PICT 2019-04329]; Secretaría de Ciencia yTecnología-Universidad Nacional de Córdoba [33620180101257CB].