Anticancer potential of Origanum munzurense extract against MCF-7 breast cancer cell

ABSTRACT

Origanum munzurense (O. munzurense) is an endemic species of Tunceli region of Turkey. In this study, we investigated in vitro anticancer effect of aqueous extract of O. munzurense (OME) on breast cancer cells (MCF-7). In vitro cytotoxic effect of OME was evaluated in MCF-7 cells by MTT assay. The wound-healing assay was used to examine the inhibition of migration. Annexin V/propidium iodide staining, cell-cycle distribution was assessed by flow cytometry for MCF-7 cells treated with OME. MTT results show that OME demonstrated in vitro cytotoxicity with 600 mg at 48 h on MCF-7. Doses of 400 µg/mL and 600 µg/mL OME significantly suppressed the migration rate of MCF-7 cells. OME significantly decreased the percentage of live cancer cells and showed an apoptotic effect after 48 h of incubation. These results show that OME is effective against breast cancer when administered at high doses and for a long time.

KEYWORDS:

• Origanum munzurense
• MCF-7
• cytotoxicity
• apoptosis
• anticancer

Highlights

• Anticancer effect of Origanum munzurense extract on breast cancer cells was investigated.

• Soxhlet extraction method was performed to obtain aqueous extract of Origanum munzurense.

• MTT assay was used to evaluate in vitro cytotoxic effect of OME in MCF-7 cells.

• The inhibition of migration was examined by the wound-healing assay.

• Extracts decreased the percentage of live cancer cells and showed an apoptotic effect.

Abbreviations

FBS: fetal bovine serumMCF-7: human breast cancer cell lineMTT: 3-4,5-dimethyl-thiazole-2,5-diphenyltetrazolium bromideOME: extract of Origanum munzurensePBS: phosphate buffer saline

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Notes on contributors

Şeyma Gülnaz Yarlılar

Şeyma Gülnaz Yarlılar Writing, reviewing & editing. Formal analysis, discussing the results, commenting on the manuscript and cell culture analysis.

Erdal Yabalak

Erdal Yabalak Writing , reviewing & editing. Formal analysis, discussing the results and commenting on the manuscript , visualisation. Preparing the plant and performing the extraction.

Derya Yetkin

Derya Yetkin: Cell culture analysis.

A. Murat Gizir

A. Murat Gizir Review of the initial manuscript

Birgül Mazmancı

Birgül Mazmancı Designing and supervising Commenting on the manuscript. All authors were informed of the content of the manuscript and agreed to submit it.