Potential Protective Effects of Ruta Chalepensis L. Extracts Against Gentamicin-Induced Nephrotoxicity via Reduction on Apoptotic, DNA Damage and Oxidative Stress Markers in Mice

ABSTRACT

In this study, the protective effects of Ruta chalepensis L. extracts on the extent of tissue damage in gentamicin-induced nephrotoxicity have been investigated. Ruta chalepensis L. extracts were prepared by subcritical water and ultrasound-assisted organic solvent extraction methods. Protective activity of Ruta chalepensis L. extracts on Gentamicin-induced nephrotoxicity is investigated by apoptotic, DNA damage, oxidative stress markers and evaluating histopathological in kidney tissue of mice. Gentamicin significantly increased Caspase-3 and −8 activities, NO levels, serum creatinine and BUN, while 8-OHdG and MDA levels were significantly decreased with Ruta chalepensis L. extract treatment. In addition, Ruta chalepensis L. extracts treatment significantly increased CAT and SOD activities. Histopathological alterations in Gentamicin group were significantly diminished by application of Ruta chalepensis L. extracts. These results suggest that treatment with Ruta chalepensis L. extracts may ameliorate renal dysfunction and structural damage through the reduction of oxidative stress and apoptosis in the kidney.

KEYWORDS:

• Nephrotoxicity
• gentamicin
• oxidative stress
• apoptosis
• Ruta chalepensis l

Author contribution

MY, ME, UD, AY, MBU, MEG and OD performed experiments and writing the manuscript; MY and ESG analyzed the data; MY, ME and ESG designed the study and edited the manuscript. The authors declare that all data were generated in-house and that no paper mill was used.

Disclosure statement

No potential conflict of interest was reported by the author(s).

Ethics approval

The study was approved by the Ethics Committee Harran University/Turkey (protocol number: 2021/007/02).

Additional information

Funding

The author(s) reported that there is no funding associated with the work featured in this article.