Apoptotic and antiproliferative effects of Inula viscosa L. water extract in the expression of microRnas on HCT 116 cell line: an in vitro study

ABSTRACT

In this study, the antiproliferative and apoptotic effects of Inula viscosa L. water extract (IVE) on HCT 116 has been examined, and the change in the expression of miRNAs. Phenolic compounds of IVE were determined as µg/g extract using by HPLC‐DAD. Quantitative determination of apoptosis, cell viability, IC50 values and miRNAs of the cells were determined during 24, and 48 hours. IVE contain coumarin, rosmarinic acid and chlorogenic acid. According to the findings of our study, the expression of miR-21 and miR-135a1 was upregulated, and miR-145 was downregulated in HCT 116 cells (Control). Additionally, IVE was found to have significant potential in regulating miRNAs, downregulating miR-21, miR-31 and miR-135a1, and upregulating miR-145 in HCT-116 cells. All these results show that the anticancer effect of IVE via regulating miRNAs’ expression has been demonstrated for the first time, and may be candidate biomarkers in colorectal cancer.

KEYWORDS:

• Bioactive compounds
• cell
• colorectal cancer
• Inula viscosa L.
• miRNA

Acknowledgements

Apaydin Yildirim B study design, literature review, statistical analysis of data, biochemical and molecular analysis; Gedikli S determined apoptosis by performing immunohistochemical analysis; Kordali S contributed to the study in the collection and extraction of the plant. Selcuk Kucukaydin was determinated of phenolic composition of IVE. All authors approved the publication by checking the final version of the article.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

The work was supported by the Ataturk University Scientific Research Foundation [TCD-2019-7446]