Supplemental feeding of a gut microbial metabolite of linoleic acid, 10-hydroxy-cis-12-octadecenoic acid, alleviates spontaneous atopic dermatitis and modulates intestinal microbiota in NC/nga mice

Abstract

The present study investigated the antiallergic and anti-inflammatory effects of 10-hydroxy-cis-12-octadecenoic acid (HYA), a novel gut microbial metabolite of linoleic acid, in NC/Nga mice, a model of atopic dermatitis (AD). Feeding HYA decreased the plasma immunoglobulin E level and skin infiltration of mast cells with a concomitant decrease in dermatitis score. HYA feeding decreased TNF-α and increased claudin-1, a tight junction protein, levels in the mouse skin. Cytokine expression levels in the skin and intestinal Peyer’s patches cells suggested that HYA improved the Th1/Th2 balance in mice. Immunoglobulin A concentration in the feces of the HYA-fed mice was approximately four times higher than that in the control mice. Finally, denaturing gradient gel electrophoresis of the PCR-amplified 16 S rRNA gene of fecal microbes indicated the modification of microbiota by HYA. Taken together, the alterations in the intestinal microbiota might be, at least in part, associated with the antiallergic effect of HYA.

Graphical Abstract

Keywords:

• 10-Hydroxy-cis-12-octadecenoic acid
• gut microbial metabolite
• atopic dermatitis
• intestinal microbiota

Acknowledgements

This research was supported partially by grants-in-aid from the Japanese Ministry of Education, Science, Sports and Culture to S. Tanabe, from the Bio-Oriented Technology Research Advancement Institution of Japan to J. Ogawa, from the Science and Technology Promotion Program for Agriculture Forestry, Fisheries, and Food Industry of the Ministry of Agriculture, Forestry, and Fisheries of Japan to J. Ogawa, from the Advanced Low Carbon Technology Research and Development Program of Japan to S. Kishino, and from the NEDO Innovation Commercialization Venture Support Project in collaboration with NITTO PHARMA and J. Ogawa.

Disclosure statement

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of this manuscript.

Additional information

Funding

This research was supported partially by grants-in-aid from the Japanese Ministry of Education, Science, Sports and Culture to S. Tanabe, from the Bio-Oriented Technology Research Advancement Institution of Japan to J. Ogawa, from the Science and Technology Promotion Program for Agriculture Forestry, Fisheries, and Food Industry of the Ministry of Agriculture, Forestry, and Fisheries of Japan to J. Ogawa, from the Advanced Low Carbon Technology Research and Development Program of Japan to S. Kishino, and from the NEDO Innovation Commercialization Venture Support Project in collaboration with NITTO PHARMA and J. Ogawa.