Resveratrol inhibits the proliferation of melanoma cells by modulating cell cycle

Abstract

The aim of this study was to evaluate the inhibitory effects of resveratrol (RSV) in A375 and A431 melanoma cells, by assessing cell viability (CCK-8 assay), apoptosis through flow cytometer and cell morphology, cell cycle assay by flow cytometer and western blot (Cyclin D1, Rac1 and PCDH9). Our results demonstrated that RSV strongly inhibited A375 cells proliferation, by decreasing cell viability, promoting apoptosis and arresting cell cycle. Besides, to clarify the main factor – duration or concentration of RSV, the double variance analysis of independent factors was operated after Bartlett’s test for homogeneity by R project. According to the outcomes obtained from statistical analyses, Cyclin D1 and PCDH9 were strongly affected by RSV duration while Rac1 was not influenced. In conclusion, RSV can inhibit A375 proliferation and trigger apoptosis by influencing cell cycle proteins; for these effects, treatment duration of RSV played more important role than concentration.

Keywords:

• Melanoma
• resveratrol (RSV)
• apoptosis
• cell morphology
• cell cycle

Disclosure statement

The authors have declared that there are no conflicts of interest.

Additional information

Funding

This research was supported by Natural Science Foundation of Guangdong Province of China [2016A03031368]; National Student’s Platform for Innovation and Entrepreneurship Training Program [201810571017]; Guangdong Medical University College Students Innovation Experiment Project [2016ZZZF003].