Comparative efficacy of indigenous heterorhabditid nematodes from north western Himalaya and Heterorhabditis indica (Poinar, Karunakar & David) against the larvae of Helicoverpa armigera (Hubner)

ABSTRACT

The efficacy of three Heterorhabditis spp of entomopathogenic nematodes (EPNs) from north western Himalaya, India was studied against the larvae of pod borer, Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae), under the laboratory conditions. The larvae were exposed to 10, 20, 30 and 40 infective juveniles (IJs) of each nematode species for different time periods and they were found to be susceptible to all the EPNs tested. However, the susceptibility of larvae to nematode infection varied according to the concentrations of IJs and their exposure periods. The efficacy of these indigenous entomopathogenic nematodes was also compared against commercially available entomopathogenic nematode, H. indica. Appreciably good performance was achieved by H. bacteriophora (HRJ), which showed 73.3% mortality of insect larvae in 96 h exposure time against third instar larvae, while H. indica produced 80.0% mortality. However it was noticed that with the advancement of larval stage its mortality rate reduced and vice versa with the exposure period. All tested EPNs were also found to reproduce within the host and maximum production of IJs was recorded in H. bacteriophora (26.0 ± 3.76 × 10³ IJs/larva) at the concentration of 40 IJs/larva.

KEYWORDS:

• Helicoverpa armigera
• biological control
• Heterorhabditis
• entomopathogenic nematodes
• virulence

Disclosure statement

No potential conflict of interest was reported by the authors.