Genetic polymorphisms in KCNJ11 (E23K, rs5219) and SDF-1β (G801A, rs1801157) genes are associated with the risk of type 2 diabetes mellitus

Abstract

Background

Type 2 diabetes mellitus (T2DM) is a global major health problem resulting from interaction of environmental and genetic factors, examples of the latter being KCNJ11 (coding for part of the ATP-sensitive potassium channel) and SDF-1β (coding for chemokine CXCL12). Our case-control study was conducted to assess whether recessive, dominant or additive genotype model associations of KCNJ11 (E23K, rs5219) and SDF-1β (G801A, rs1801157) were more strongly linked to type 2 diabetes.

Subjects & Methods

Genetic polymorphism analysis was performed by polymerase chain reaction-restriction fragment length polymorphism. Alleles and genotype frequencies between 200 cases and 200 controls were determined and compared.

Results

The dominant (EE v EK + KK, p = 0.022) and additive (EK v EE + KK, p = 0.021) models, but not the recessive model (KK v EE + EK, p = 0.727) of KCNJ11 were linked to diabetes. Similarly, the dominant (GG v GA + AA, p < 0.001) and additive (AG v GG + AA, p=<0.001) models, but not the recessive model (AA v AG + GG, p = 0.430) of SDF-1β were linked to diabetes. The A allele (p = 0.006) of SDF-1β was protective against the risk of T2DM.

Conclusion

Both dominant and additive models in both KCNJ11 (E23K, rs5219) and SDF-1β (G801A, rs1801157) genetic polymorphisms are significantly associated with type 2 diabetes.

Keywords:

• Gene polymorphism
• KCNJ11
• SDF-1β
• type 2 diabetes mellitus

Acknowledgement

We would like to acknowledge the entire Medical and Research staff and our colleagues at Era’s Lucknow Medical College and Amity University for their support and contributions in this study. This work will be used by Saliha Rizvi for partial fulfillment of the degree requirement for her doctoral research at Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow.