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Abstract
Saifi, H. W., Bhushan, B., Kumar, S., Kumar, P., Patra, B. N. and Sharma, A. 2005. Estimation of genetic distance between Bhadawari and Murrah buffaloes (Bubalus bubalis) using band sharing frequency. J. Appl. Anim. Res., 27: 11–15.
RAPD-PCR analysis was conducted on Bhadawari and Murrah breeds of buffaloes using 18 random primers out of which 11 primers yielded distinct RAPD profiles. Primers OPA01, OPG05, OPA14 and BG15 generated relatively higher band sharing frequency (BSF) ranges from 0.553 to 1.00 within and between two breeds. The BSF pooled over 11 primers were 0.724±0.049 within Bhadawari and 0.675±0.048 within Murrah breeds, whereas it was 0.566±0.045 between the two breeds. Primer OPA04 showed highest (0.720) genetic distance (D) between these two breeds. The overall genetic distance (0.219±0.071) based on BSF indicated lower degree of genetic divergence between Bhadawari and Murrah. It may be concluded that the overall genetic distance, based on BSF indicated the lower degree of genetic divergence between Bhadawari and Murrah buffaloes.