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Original Articles

Molecular Method of Sex Identification in Siberian White Crane (Grus eucogeranus)

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Pages 169-172 | Received 21 Aug 2008, Accepted 01 Feb 2009, Published online: 15 Nov 2011
 

Abstract

Bao, W.B., Musa, H.H., Luan, D.Q., Zhang, H.X. and Chen, G.H. 2009. Molecular method of sex identification in Siberian White crane (Grus eucogeranus). J. Appl. Anim. Res., 35: 169–172.

For sex identification nondestructive sampling method was used to collect feathers from Siberian White crane to extract genomic DNA. Thereafter, three combinations of primers were used to amplify the EE0.6 sequence. The male birds displayed only one distinct amplified band, whereas, females displayed two bands. The results concluded that PCR technique for sex-related gene amplification is successful for sex identification of Siberian White crane. This method may also guide for accurate sex identification of other endangered birds.

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