β-Tubulin Gene Based PCR-RFLP Method for Specific Detection of Babesia bigemina and Theileria annulata Isolates

Abstract

Singh, H., Cheema, P.S., Mishra, A.K., Tewari, A.K., Rao, J.R., Ravindran, R., Ray, D. and Bansal, G.C. 2010. β-tubulin gene based PCR-RFLP method for specific detection of Babesia bigemina and Theileria annulata isolates. J. Appl. Anim. Res., 37: 233–238.

For early detection of Babesia bigemina and Theileria annulata β-tubulin gene was targeted based on PCR assays. Subsequently, on the basis of sequence analysis of β-tubulin gene fragment of various isolates, a new PCR-RFLP method based on enzymes EcoRI and HindIII has been developed for species identification of B. bigemina and T. annulata isolates.

Keywords:

• Bαbesia bigemina
• β-tubulin
• cattle
• PCR-RFLP
• Theileria annulata