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Abstract
Phlomis cancellata Bunge. is a native plant belongs to Labiatae family which can used more in modern medicine and different industries for its essential oil particulars, in addition to Iranian folk medicine. In this study, P. cancellata were collected in full flowering stage from mountain of Gaduk area in Mazandaran province, Iran. The aerial parts of P. cancellata were subjected to hydrodistillation (HD) in a Clevenger-type apparatus (5 h). The yield of the oil was 0.85 % (w/w). The essential oil was analyzed by GC and GC-MS. After identification of the components 44 components were identified which contained 95.8 % of the oil. Hexadecanoic acid (17.3 %), germacrene D (14.6 %), eudesmol (8.5 %), octacosane (5.6 %), (E)-caryophyllene (5.4 %), heptacosane (4.9 %) and pentacosane (3.6 %) were major components in P. cancellata oil. In vitro antimicrobial activity of essential oil of P. cancellata against a panel of four strains of bacteria have shown larger growth inhibition zone diameters (21 and 25 mm) against the gram-positive tested bacterial strains compared with gram-negative bacteria (13 and 14 mm). Antimicrobial activities of essential oil was also tested by MIC and MBC method, utilizing four gram (+/-) microorganism, exhibited the same results (MIC 4 and 8 µg/ml and MBC 8 and 16 µg/ml, against gram (+) bacteria, and also MIC 16 and 32 µg/ml and MBC 32 and 32 µg/ml, against gram (-) bacteria).