Chemical Composition of the Volatile Fractions from Flowers, Leaves and Stems of Salvia mirzayanii by HS-SPME-GC-MS

Abstract

Volatile constituents from minimal amounts of flowers, leaves and stems of Salvia mirzayanii were obtained using a manual headspace solid phase microextraction (HS-SPME) device equipped with four types of commercially available fibers, namely polydimethylsiloxane (PDMS), polydimethylsiloxane-carboxene (PDMS-CAR), polydimethylsiloxane-divinylbenzene (PDMS-DVB) and divinylbenzene-carboxene-polydimethyl siloxane (DVB-CAR-PDMS). After selection of the best-performing fiber (PDMS-CAR) and optimization of the corresponding variables, the fiber was conditioned by placing it for 25 min in the headspace of a vial containing the powdered plant organs, immediately after which it was inserted onto injection port of the GC-MS. Chromatographic analyses were performed using DB-5 and fused silica HP-5MS capillary columns with temperature programming. This study also revealed that the most abundant compounds in both in flower and leaf volatile fractions were monoterpene hydrocarbons, whereas that of stem contained mostly oxygenated monoterpenes.

Keywords:

• Salvia mirzayanii
• headspace solid-phase microextraction (HS-SPME)
• volatile fractions
• oils
• α-pinene
• limonene