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Abstract
Herbal drugs in this category are in alternative agents and for several years they have been used all around the world. The aim of this study is to examine the antimicrobial properties of three species of Punica granatum on Candida albicans and Escherichia coli and to compare it to some common antibiotics. Firstly the extracts of P. granatum var. pleniflora, P. granatum var. Sweet Alak, P. granatum var. Saveh Black leather and leaf Portulaca olearaceae were prepared and the amount of total phenol and free radical scavenging activity had been measured in the plants. Next the antimicrobial activity of prepared extracts was determined on Candida albicans and Escherichia coli and it was compared to some common antibiotics. Also MIC50, MIC90, MFC and MBC of extracts were determined. The statistical analysis of data for checking inhibition zone (IZ) was done using Factorial test in random design and means comparison was done via Tokey method. Results showed that the high concentration of treatment P. granatum var. pleniflora had the most effect on IZ (P<0.0001) and the highest amounts of phenol and antioxidant activity is related to P. granatum var. pleniflora flower. The comparison of antibiotics and herbal extracts on Candida albicans and Escherichia coli showed that concentration 30 % of P. granatum var. pleniflora has a more significant potential compared to Amfoterisin B and antibacterial drugs. From the obtained results, it is clear that the methanolic extract of P. granatum flower can be used against Candida albicans and Escherichia coli in different concentrations. It is suggested to conduct other experiments in alternative medicine and to use the extract on different microbial species.