The microbiological hydroxylation of patchoulol in Absidia coerulea and Mucor hiemalis

Abstract

Patchoulol was subjected to transformation by Absidia coerulea AM93 and Mucor hiemalis AM450 strains. Both micro-organisms, which displayed differing vulnerability to the fungistatic action of the patchoulol, were capable of selective hydroxylations of the substrate. The major constituents of the mixtures after transformation were (8S)-8-hydroxypatchoulol and (9R)-9-hydroxypatchoulol; the transformation carried out by M. hiemalis resulted in the additional formation of (3R)-3-hydroxypatchoulol. The mixture of (8S)-8-hydroxypatchoulol and (9R)-9-hydroxypatchoulol can be used in the synthesis of patchoulenol, a compound with an odour very similar to that of a valuable fragrance, norpatchoulenol.

• Biotransformation
• hydroxylation
• patchoulol
• Absidia coerulea
• Mucor hiemalis
• antifungal activity