Enzymatic synthesis of gallic acid from tannic acid with an inducible hydrolase of Enterobacter spp

Abstract

Gallic acid acts as a precursor molecule to synthesize various tannin molecules. These are plant polyphenols and were proved to be good anti-oxidant, anti-cancerous, anti-inflammatory, anti-microbial compounds. In order to fully exploit prominent biological activities of specific tannins and to develop tannin-based new medicines, it is necessary to obtain their pure preparations with an aim of high yield and specificity. In the present study, gallic acid is synthesized by the hydrolysis of tannic acid using a microbial based transformation process. The microorganism was isolated and identified. The ability of the isolated microorganism to covert tannic acid into gallic acid was determined by HPLC and enzyme production.

Highlights

• The present investigation signifies the role of Enterobacter spp. in various processes:

• • To synthesize gallic acid (a precursor for food oxidant such as propyl gallate) and a bacteriostatic antibiotic (trimethoprim).

• • To protect the environment from tannery’s discharge through the process of biodegradation.

• • To reduce the toxicity of tannins in animal feed.

Keywords:

• Tannic acid
• gallic acid
• tannase
• biotransformation
• PCR
• BLAST
• HPLC

Acknowledgements

The authors acknowledge the help and guidance given by Dr. R. S. Jayani and Dr. Satyendra Kumar in identifying and characterizing the organism. Assistance given by Mr. Priyadarshi Meena, Ph.D. Scholar in the Department of Zoology, University of Rajasthan is greatly acknowledged.

Disclosure statement

All others contributed equally in the preparation of manuscript. We hereby declare that we have no conflict of interest in the submission of manuscript entitled “Enzymatic Synthesis of Gallic acid from Tannic acid with an Inducible Hydrolase of Enterobacter spp.” for the publication in Biocatalysis and Biotransformation.