Microbial transformation of androstenedione by Cladosporium sphaerospermum and Ulocladium chartarum

Abstract

In this work, incubations of androstenedione 1 with Cladosporium sphaerospermum MRC 70266 and Ulocladium chartarum MRC 72584 have been reported. C. sphaerospermum MRC 70266 mainly hydroxylated 1 at C-6β, accompanied by a hydroxylation at C-15α, a reduction at C-17, a 5α-reduction and oxidations at C-6 and C-16 following hydroxylations. U. chartarum MRC 72584 hydroxylated 1 at C-6β, C-7α, C-7β and C-14α, accompanied by an oxidation at C-6 following its hydroxylation, a reduction at C-17 and a 5α-reduction. 6β,17β-Dihydroxyandrost-4-en-3,16-dione 8, one of the metabolites from the incubation of 1 with C. sphaerospermum MRC 70266, was determined as a new compound.

Keywords:

• Cladosporium sphaerospermum
• Ulocladium chartarum
• androstenedione
• biotransformation

Acknowledgements

We are grateful to Rasit Yilmaz, Yavuz Derin and Fatih Sonmez for running the ¹H and 13C NMR spectra.

Disclosure statement

The authors report no declaration of interest. The authors alone are responsible for the content and writing of the article.

Additional information

Funding

This work was financially supported by the Sakarya Üniversitesi Scientific Research Foundation (Project no. 20182719), Turkey.