Non-isothermal kinetic modelling of potassium indigo-trisulfonate dye discolouration by Horseradish peroxidase

Abstract

Textile industries account for two-thirds of the total dyestuff market been responsible for the production of a large volume of effluent with unfixed dye. Aromatic dyes as potassium indigo-trisulfonate dye (PIT) are characterized as a chemically stable and complex molecule with high heat and light stability and with high toxicity even at low concentration. In order to evaluate an environmentally friendly method to remove potassium indigo-trisulfonate dye from aqueous solution, a commercial peroxidase (horseradish peroxidase, HPR) was used in the experimental and the data were modelled by the Arrhenius equation. According to the results, the best reaction conditions were obtained using 80 mg.L⁻¹ of dye concentration, 45 °C, pH 5.0, 349.35 U.mL⁻¹, and 4.5 mM hydrogen peroxide concentration leading to a 96% of dye discolouration.

Keywords:

• Peroxidase enzyme
• discolouration
• Arrhenius equation
• kinetic constants
• modelling

Acknowledgements

The authors are grateful to the Toyobo do Brazil Company for the HRP enzyme. The Interdisciplinary Laboratory for the Development of Nanoestructures (LINDEN/UFSC) and the staff assistance in the laboratories is gratefully acknowledged.

Disclosure statement

No potential conflict of interest was reported by the authors.

Correction Statement

This article has been republished with minor changes. These changes do not impact the academic content of the article.

Additional information

Funding

The authors are grateful for the financial support from Brazilian agencies CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior).