Bioprospecting fruit waste to purify invertase for low calorie food products

Abstract

EkINV and BsINV are two invertases from Enterobacter kobei and Bacillus subtillis, respectively. The two enzymes were produced extracellularly at pH 3.0 and former at 45 °C while latter at 25 °C. The K_m values were observed to be 1.7 mol/L (EkINV) and 1.9 mol/L (BsINV). Purification and characterization analysis revealed trimer structure EkINV having subunit molecular mass as 87.14, 42.98 and 29.0 kDa. Unlike, BsINV was monomer with molecular mass of 55.1 kDa. The invertases beside hydrolytic activity exhibited transfructolysation which is used in preparation of low calorie Ready To Serve mango juice and muffins.

Keywords:

• Invertase
• purification
• SDS-PAGE
• invert sugar
• low calorie food

Acknowledgments

Dr. Yashwant Singh Parmar, University of Horticulture and Forestry has been acknowledged as externally funding agency.

Disclosure statement

No potential conflict of interest was reported by the author(s).