L-threo 3,4-dihydroxyphenylserine treatment during mouse perinatal and rat postnatal development does not alter the impact of dietary copper deficiency

Abstract

Dietary copper (Cu) deficiency was induced perinatally in Swiss Albino mice and postnatally in male Holtzman rats to investigate the effect of L-threo 3,4-dihydroxyphenylserine (DOPS) on pup survival and catecholamine levels in a 2×2 factorial design. Mouse dams were placed on one of four treatments 14 days after mating and rats at postnatal day 19 (P19). Treatments were Cu-adequate (Cu+) and Cu-deficient (Cu−) diets with or without DOPS (1mg/ml) in the drinking water. Mouse pups were killed at P14 and rats at P49. Mortality in Cu− pups was 46% and not significantly improved by DOPS, 39%. A repeat study with mice adding ascorbic acid in the water with DOPS showed no improvement. Compared to Cu+ animals, Cu− animals were smaller, anemic and had a 92% reduction in liver Cu. DOPS treatment made no improvement to and in some cases exacerbated the Cu deficiency. Catecholamine levels measured in heart and brain by LCEC showed decreased NE levels and increased DA levels in Cu− animals compared to controls. DOPS treatment did not alter this pattern. Although DOPS was present in treated animal's tissues, survival in mice and catecholamine levels in mice and rats were not altered by the 1mg/ml dose of DOPS.

Keywords:

• L-threo 3,4-dihydroxyphenylserine
• catecholamines
• copper deficiency
• development
• mice
• rats