Abstract
Two new triterpene saponins, mandshunosides A and B (1 and 2), were isolated from the roots and rhizomes of Clematis mandshurica. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. Compounds 1 and 2 showed inhibitory activities against two colorectal human cancer cells HCT 116 (IC50 2.1 μM for 1 and 2.5 μM for 2) and HT-29 (IC50 3.7 μM for 1 and 3.3 μM for 2).
Acknowledgements
This work was financially supported by the Research Fund of Key Laboratory of TCM Biotechnology, Xihua University, and the Open Research Fund of Key Laboratory of Food Biotechnology, Xihua University (SZJJ2009-006).