Cytotoxic triterpene saponins from Clematis mandshurica

Abstract

Two new triterpene saponins, mandshunosides A and B (1 and 2), were isolated from the roots and rhizomes of Clematis mandshurica. Their structures were elucidated on the basis of spectroscopic evidence and hydrolysis products. Compounds 1 and 2 showed inhibitory activities against two colorectal human cancer cells HCT 116 (IC₅₀ 2.1 μM for 1 and 2.5 μM for 2) and HT-29 (IC₅₀ 3.7 μM for 1 and 3.3 μM for 2).

Keywords:

• Clematis mandshurica
• triterpene saponins
• mandshunoside A
• mandshunoside B
• cytotoxicity

Acknowledgements

This work was financially supported by the Research Fund of Key Laboratory of TCM Biotechnology, Xihua University, and the Open Research Fund of Key Laboratory of Food Biotechnology, Xihua University (SZJJ2009-006).