Toxicokinetics of naringin and its metabolite naringenin after 180-day repeated oral administration in beagle dogs assayed by a rapid resolution liquid chromatography/tandem mass spectrometric method

Abstract

Toxicokinetic characteristics of naringin and its metabolite naringenin were investigated in beagle dogs after oral administration of naringin at the doses of 20, 100, or 500 mg/kg/day in a repeated-dose study for 1, 30, 90, and 180 days. Plasma concentrations of naringin and naringenin were determined by a rapid resolution liquid chromatography/electrospray ionization/tandem mass spectrometric method. The results showed that no differences in systemic exposure were observed between male and female beagle dogs. Systematic exposure exhibited dose-dependent increase for both naringin and naringenin. No significant accumulations were observed. Results would be taken into consideration for the interpretation of toxicology findings and provide a reference for clinical safety assessment.

Keywords:

• toxicokinetics
• naringin
• naringenin
• beagle dogs

Acknowledgments

This work was jointly supported by the National Key Technology R&D Program (No. 2006BAI06A02-2), the Guangdong-Hongkong Major Breakthrough Project of Key Realms (No. 2009A030901009), and the National Major Scientific and Technical Special Project (No. 2011ZX09102-011-03).

Notes

1. These authors contributed equally to this work.

Additional information

Notes on contributors

Cui-Ping Yang

1

Meng-Hua Liu

1