A new 3,4-seco-labdane diterpenoid with inhibitory activity against the production of nitric oxide from the leaves of Callicarpa nudiflora

Abstract

A new 3,4-seco-labdane diterpenoid, 3,4-seco-12R,13S-dihydroxy-4(18),8(17),14(15)-labdatrien-3-oic acid (1), was isolated from the leaves of Callicarpa nudiflora. The structure of 1 was elucidated by 1D NMR, 2D NMR and HRESIMS data, and confirmed by single-crystal X-ray diffraction analysis. 3,4-seco-12R,13S-Dihydroxy-4(18),8(17),14(15)-labdatrien-3-oic acid (1) exerted inhibitory activity against the production of nitric oxide in RAW264.7 stimulated by lipopolysaccharide.

Keywords::

• 3,4-seco-labdane diterpenoid
• Callicarpa nudiflora
• X-ray diffraction
• nitric oxide

Acknowledgements

Financial support of National Natural Science Foundation (Grant No. 81060367) and National Key Technology R&D Program (Grant No. 2011BAI01B07) are gratefully acknowledged. We thank for Ms Wen Li and Mr Yi Sha of Analysis & Testing Centers of Shenyang Pharmaceutical University for the NMR data measurements. We gratefully acknowledge the Hainan Jiuzhitang Co., Ltd for the collection of the plant material. We also thank Professor Weiping Chen of Chinese Academy of Medical Sciences, Hainan Union Medical College Institute of Medical Plant Development for identification of the plant material.