Abstract
Two new 20α-ursane-type triterpenoids (1–2) were isolated from the roots of Ilex cornuta, along with three known triterpenoids (3–5). The structures of compounds 1–2 were determined as 3β, 23-dihydroxy-20α(H)-urs-12-en-28-oic acid (1), 3β,19α,23-trihydroxy-20α(H)-urs-12-en-28-oic acid 3β-O-α-l-arabinopyranoside (2), on the basis of hydrolysis and spectral evidence, including 1D- and 2D-NMR and high-resolution electrospray ionization mass spectrometry analyses. These pure isolates (1–5) were tested for their cytotoxic activities by MTT assay.
Acknowledgments
This paper was funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We also thank Mrs Li-Ping Shi from the Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences for the determination of NMR spectral data.