Two new triterpenoid saponins from Ilex cornuta

Abstract

Two new triterpenoid saponins (1 and 2) were isolated from the stems of Ilex cornuta, along with two known triterpenoids (3 and 4). The structures of compounds 1 and 2 were determined as ursane-12,19-diene-28-oic acid 3β-O-β-d-glucuronopyranoside-6-O-methyl ester (1), 3α,23α-dihydroxy-olean-9(11),12-diene-28-oic acid 28-O-β-d-glucopyranosyl ester (2), on the basis of hydrolysis and spectral evidence, including 1D and 2D NMR and high resolution electrospray ionization mass spectrometry (HR-ESI-MS) analyses. Protective effects of compounds 1–4 were tested against H₂O₂-induced H9c2 cardiomyocyte injury, and the data showed that all these compounds had no cell-protective effect.

Keywords::

• Ilex cornuta
• Aquifoliaceae
• ursane-12
• 19-diene-28-oic acid 3β-O-β-d-glucuronopyranoside-6-O-methyl ester
• 3α
• 23α-dihydroxy-olean-9(11)
• 12-diene-28-oic acid 28-O-β-d-glucopyranosyl ester
• cardiomyocyte oxidative injury
• cell-protective effect

Disclosure statement

No potential conflict of interest was reported by the authors.