Discovery, gene modification, and optimization of fermentation of an enduracidin-producing strain

Abstract

Enduracidin significantly inhibits Gram-positive bacteria and had been widely used in many fields. However, as the poor technology for production of enduracidin and its scarcity, identification of novel strategies for production of enduracidin is important. Our group developed two methods to improve the yield of the production of enduracidin. The yield of enduracidin was increased by three- to fivefold. The highest yields of enduracidin A and enduracidin B achieved were 63.7 and 82.13 mg/ml. Thus, our results might provide a new reference method for the industrial production of enduracidin.

Keywords:

• Enduracidin
• gene mutation
• fermentation optimization
• marine microorganisms

Acknowledgments

Beside, I would like to express my gratitude for MCCC to provide strains and Scientific Instruments Sharing Platform (Third Institute of Oceanography) to provide technical support.