Dendrodoristerol, a cytotoxic C20 steroid from the Vietnamese nudibranch mollusk Dendrodoris fumata

Abstract

Using various chromatographic separations, four steroids including one new C₂₀ steroid namely dendrodoristerol (1), were isolated from the Vietnamese nudibranch mollusk Dendrodoris fumata. The structure elucidation was confirmed by combination of spectroscopic experiments including 1D and 2D NMR, HR QTOF MS, and CD. Compound 1 was found to exhibit significant in vitro cytotoxic activity against six human cancer cell lines as HL-60, KB, LU-1, MCF-7, LNCaP, and HepG2. In addition, 1 induced HL-60 cancer cell death by apoptosis and necrosis.

Graphical Abstract

KEYWORDS:

• Dendrodoris fumata
• steroid
• cytotoxicity
• apoptosis
• necrosis

Acknowledgments

The authors are grateful to the Institute of Chemistry, VAST for measurement of the NMR spectra and Dr. Bui Huu Tai, Institute of Marine Biochemistry, VAST for measurement of the CD and HR-QTOF mass spectra.

Disclosure statement

No potential conflict of interest was reported by the authors.

Supporting information

HR-QTOF mass, CD, 1D and 2D NMR spectra for the new compound 1; Hoechst staining, Flow cytometry analysis apoptosis, and Caspase 3 activity.

Additional information

Funding

This study was financially supported by a grant from Vietnam Academy of Science and Technology (code: VAST.TĐ.DLB.06/16-18).