Two new prenylated coumarins from roots of Zanthoxylum nitidum

Abstract

Two new prenylated coumarins, 3'-hydroxytoddanone (1), and isotoddalolactone (2), along with four known analogues (3–6) were isolated from the roots of Zanthoxylum nitidum. Their chemical structures were elucidated based on extensive spectroscopic interpretation and HR-ESI-MS analysis. The absolute configuration of compound 2 was determined by comparing experimental ECD spectrum with that calculated by the time-dependent density functional theory (TDDFT) method. Compounds 4–6 were isolated from the Zanthoxylum genus for the first time. The two new compounds were tested for antiproliferative activities in vitro on the HL-60, K562 and THP-1 cell lines. Compounds 1 and 2 exhibited moderate cell growth inhibitory activities in vitro against human leukemic HL-60 cell lines, with IC₅₀ values of 32.64 and 33.15 µM, respectively.

Graphical Abstract

Keywords:

• Rutaceae
• Zanthoxylum nitidum
• coumarin
• NMR
• antiproliferation

Acknowledgements

The authors are grateful to Mr. Jing-Chao Liu and Mr. Zi-Shuang Liu (Analytical Testing Center, Northeast University, Shenyang, China) for their measurements of the NMR data.

Disclosure statement

No potential conflict of interests was reported by the authors.

Additional information

Funding

This work was financially supported by the Scientific Research Foundation of Shenyang Medical College (No. 20191022), Undergraduate Scientific Research Projects of Shenyang Medical College (No. 20209033) and Scientific Research Foundation of Shenyang Agricultural University (No. 880415026).