Sulfation of hyperoside by the human cytosolic sulfotransferases (SULTs): impact of genetic polymorphisms on hyperoside-sulfating activity of SULT1C4 allozymes

Abstract

This study aimed to identify human cytosolic sulfotransferases (SULTs) that are capable of mediating hyperoside sulfation and examine the impact of genetic polymorphisms on their sulfating activity. Of the thirteen known human SULTs analyzed, five (1A1, 1A2, 1A3, 1C2, and 1C4) displayed sulfating activity toward hyperoside. Kinetic parameters of SULT1C4 that showed the strongest sulfating activity were determined. Ten SULT1C4 allozymes previously prepared were shown to display differential sulfating activities toward hyperoside, revealing clearly the functional impact of SULT1C4 genetic polymorphisms. These findings provided a robust biochemical foundation for further studies on the metabolism of hyperoside by sulfation.

Graphical Abstract

Keywords:

• Hyperoside
• sulfation
• cytosolic sulfotransferase
• SULT
• single nucleotide polymorphiosm
• SNP

Acknowledgments

We wish to thank Dr. Katsuhisa Kurogi of the University of Miyazaki for his kind advice in the preparation of this manuscript.

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This work was in part supported by the funds from China Scholarship Council; the cooperative project on scientific research between Nanchong city and North Sichuan Medical College (Grant number: 18SXHZ0250).