Two new flavonoid thioglucosides from the seeds of Lepidium apetalum

Abstract

Two previously undescribed flavonoid thioglucosides lepidiumflavonosides A and B (1–2) and two known megastigmane compounds (7E,9S)-9-hydroxy-5,7-megastigmadien-4-one 9-O-β-D-glucopyranoside (3) and (9S)-4-oxo-β-inol β-D-glucopyranoside (4) were isolated from the water extract of the seeds of Lepidium apetalum Willd. The structural elucidation of isolated compounds was unambiguously determined based on extensive 1D and 2D NMR spectroscopic analyses. All compounds were evaluated for their estrogen-like effects on MCF-7 cells in vitro. The results showed that compounds 1–4 significantly promoted the proliferation of MCF-7 cells, and the proliferation was antagonized by the specific ER antagonist ICI182,780, suggesting that compounds 1–4 might have the estrogen-like effect in vitro potentially.

Graphical Abstract

Keywords:

• Brassicaceae
• Lepidium apetalum
• seeds
• flavonoid thioglucosides
• estrogen-like effect

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was funded by the National Basic Research Program of China [2019YFC1708802], National Natural Science Foundation of China [82104386], the Scientific and Technological Key Project in Henan Province [212102310345], and the Special Project of Scientific Research on Traditional Chinese Medicine in Henan [20-21ZY2151].