Characterization and identification of major flavonoids of bamboo leaf extract by HPLC/ESI-QTOF-MS/MS

Abstract

Bamboo leaf extract (BLE) is a pale brown powder extracted from bamboo leaves, and it is listed in the Chinese Standard GB-2760 as a legal and safe food additive. The present study aims to identify and characterize the major flavonoids in BLE. The identification of major flavonoids was carried out using ultra performance liquid chromatography combined with electrospray ionization quadruple time-of-flight tandem mass spectrometry (HPLC/ESI-QTOF-MS/MS). A total of 31 flavonoid compounds were identified and tentatively characterized base on reference standards and MS dissociation mechanisms. HPLC/ESI-QTOF-MS can serve as an important analytical platform to identification structure of bamboo leaf flavonoids (BLF).

Keywords:

• Bamboo leaf extract (BLE)
• bamboo leaf flavonoids (BLF)
• HPLC/ESI-QTOF-MS/MS
• Pleioblastus maculatus
• fragmentation pathway

Disclosure statement

No potential conflict of interest was reported by the authors.

Additional information

Funding

This study was financially supported by the National key research and development program project [2022YFD2200905 to Xue-Feng Guo].