Schisandrin A can promote the anti-tumor effect of 5-Fu by reversing the immunosuppressive state of the body in rat

Abstract

In this study, based on Walker 256 in vitro experiments, CCK-8 assay, clone formation assay, wound healing assay, and flow cytometry were used to detect cell apoptosis and cell cycle. It was found that schisandrin may have significant anti-tumor effects in vitro by inhibiting TGF-β/Smad signaling pathway. In addition, in vivo experiments, immunohistochemistry was used to observe the expression of HIF-1α, VEGF and VEGFR-2 in tumor tissues. It was found that schisandrin could significantly improve the immunosuppression induced by 5-Fu and enhance the antitumor effect of 5-Fu. The mechanism may be related to the inhibition of Wnt-1/β-catenin signaling pathway.

Graphical abstract

Keywords:

• Schisandrin A
• 5-Fu
• hepatocellular carcinoma
• immunomodulation

Disclosure statement

No potential conflict of interest was reported by the author(s).

Additional information

Funding

This research was supported by the fund of Key Scientific Research Project of Colleges and Universities in Henan Province (23A320050; 23A360018); Henan Province Key Research and Development and Promotion Special (242102311083; 242102311261).