Novel furostanol saponins from the bulbs of Alliummacrostemon B. and their bioactivity on [Ca²⁺]_i increase induced by KCl

Abstract

Chemical reinvestigation of the ethanol extract of the dried bulbs of Allium macrostemon B. led to the isolation of two novel furostanol saponins, named macrostemonoside M (1) and macrostemonoside N (2), together with six known saponins. The structures of new compounds were elucidated on the basis of extensive spectroscopic analysis including 1D and 2D NMR as (25R)-22-hydroxy-5β-furostane-1β,2β,3β,6α-tetraol-26-O-β-d -glucopyranoside and 22-hydroxy-5β-furost-25-(27)-ene-1β,2β,3β,6α-tetraol-26-O-β-d -glucopyranoside, respectively. The pharmacological activities of all the saponins on [Ca²⁺]_i increase induced by KCl were evaluated.

Keywords:

• Allium macrostemon B
• Novel furostanol saponins
• Macrostemonoside M
• Macrostemonoside N
• [Ca²⁺]_i increase

Acknowledgements

The authors are grateful to Professor QiShi Sun of Shenyang Pharmaceutical University (Liaoning province, China) for identifying the plant materials and Xue Zhang of Shenzhen Research Centre of Traditional Chinese Medicines and Natural Products (Shenzhen, China) for measuring all NMR measurements.