Further studies on the chemical constituents of Chinese folk medicine Gentiana apiata N.E. Br.

Abstract

One new secoiridoid glycoside with conjugated diene, named 3-epi-swertiajaposide C (1), was isolated from the whole plants of Gentiana apiata N.E. Br., together with 11 known compounds, 7-deoxyloganic acid (2), isoorientin (3), gentiopicroside (4), silybin B (5), swertiamarin (6), asystasioside A (7), 6′-O-β-d-glucopyranosylgentiopicroside (8), umbelliferone (9), oleanolic acid (10), kaempferol (11), and β-sitosterol (12). The structure of the new compound (1) was elucidated on the basis of spectroscopic evidence including UV, IR, MS, NMR, HMBC, HMQC, and NOESY. Compounds 1, 2, 5, 6, 8, 9, and 11 were found in this plant for the first time. Moreover, silybin B (5) was isolated from the other plants besides Silybum marianum (L.) Gaertn for the first time by the present study.

Keywords:

• Gentiana apiata N.E. Br.
• iridoid
• secoiridoid
• 3-epi-swertiajaposide C
• silybin B
• 7-deoxyloganic acid

Acknowledgements

This work is supported by the National Natural Science Foundation of China (Nos 30571402 and 30771454) and the Natural Science Foundation of Shaanxi Province (No. 01-4531-41), China.