Scutellarin protects against lipopolysaccharide-induced acute lung injury via inhibition of NF-κB activation in mice

Abstract

This paper investigates the effect of natural scutellarin on acute lung injury (ALI) induced by Escherichia coli endotoxin lipopolysaccharide (LPS) in mice and its mechanism of action. Mouse ALI was induced by the injection of LPS (15 mg/kg) via the tail vein, and mice were intraperitoneally injected with 50 and 25 mg/kg of scutellarin before the LPS injection. The lung index, serum , and tumor necrosis factor-alpha (TNF-α) levels were determined using kits. The lung lesions were examined by light microscope. The mRNA levels of TNF-α, inducible nitric oxide synthase (iNOS), and FasL in pulmonary tissues were detected by RT-PCR. c-Fos, c-Jun, IκB, and iNOS proteins were detected by the western blotting method. Pretreatment with 25 and 50 mg/kg of scutellarin significantly reduced lung injury induced by LPS, which expressed in the decrease in lung morphological lesions, serum , TNF-α levels, lactate dehydrogenase release, and total protein in the lavage fluid of bronchoalveolar of the lung. The mRNA level of TNF-α, iNOS, the protein content of c-Fos, iNOS, and the activation of NF-κB in pulmonary tissues were all inhibited, while the lung glutathione level increased. In conclusion, scutellarin has protective action against LPS-induced lung damage in mice, and its underlying mechanism might be the inhibition of IκBα degradation and the expression of TNF-α mRNA.

Keywords:

• scutellarin
• LPS
• iNOS
• NF-κB
• acute lung injury

Acknowledgement

This work was financially supported by a grant (2003CB514128) from the Ministry of Science and Technology of China.

Notes

¹ Visiting scholar from Sichuan Institute of Chinese Materia Medica, Chengdu 610041, China.

Additional information

Notes on contributors

Zheng-Huai Tan

1 1 Visiting scholar from Sichuan Institute of Chinese Materia Medica, Chengdu 610041, China.