Constituents from the testas of Castanea mollissima Blume with α-glucosidase inhibitory activity

Abstract

In the screening of biologically active constituents from medicinal plants, the 75% EtOH extract of the testas of Castanea mollissima Blume showed potent α-glucosidase inhibitory activity. By means of various chromatographic methods, the extract gave a new dammarane-type triterpene 1 along with 17 known compounds. The structure of 1 was determined to be 3β-acetoxy-20-oxo-21-nordammaran-23-oic acid by HRMS and NMR studies including 2D NMR experiments. The new compound and some known compounds showed potent α-glucosidase inhibitory activity with acarbose as a positive control.

Keywords:

• Castanea mollissima Blume
• 3β-acetoxy-20-oxo-21-nordammaran-23-oic acid
• α-glucosidase inhibitory activity

Acknowledgements

We thank Mrs Wen Li, Ms Yi Sha, and Ms Weiming Cheng of the Analytical Centers of the Shenyang Pharmaceutical University for recording NMR spectra and HR-ESI-MS. This work was supported by the Scientific Project Research of Hebei Province in China.