Abstract
This study aimed to determine the incorporation of PAHs into muscle of Cyprinus carpio. Three model compounds (phenanthrene, pyrene and benzo[a]pyrene) were orally administered during 56 days, in a series of three increasing doses (0, 100, 500 μg of each PAHs per kg of fish). PAHs and their hydroxylated metabolites were analyzed by a HPLC-Fluorimetry method. Results of this study showed a significant increase of phenanthrene and pyrene in muscle. Benzo[a]pyrene incorporation to muscle was not effective. Concerning hydroxylated metabolites, they were not detected in muscle of carp. Steady states for phenanthrene and pyrene were obtained after 28 days of exposure. Low incorporation to muscle was observed at equilibrium for phenanthrene (6% and 3%) and pyrene (3% and 1%), depending of the dose tested.
ACKNOWLEDGEMENT
The authors would like to express sincere thanks to Marie-Antoinette Dziurla and Christine Grandclaudon for their analytical contribution. They are also grateful to Yannick LeDoré and Alain Iuretig for their technical assistance. This research was funded by the Agence de l'Eau Rhin-Meuse and the Zone Atelier Moselle which are gratefully acknowledged.