Improved Solubility of Morin Using p-sulphonatocalix[4]arene as Encapsulating Agent: HPLC Analysis and their Molecular Modelling

GRAPHICAL ABSTRACT

ABSTRACT

This study reports the use of water-soluble p-sulphonatocalix[4]arene (pSC-3) as solubilizing agent to improve the water solubility of morin, an exceptional antioxidant. Stoichiometry, stability constant, and mechanism of complex were investigated by UV-Vis and HPLC analysis and characterized by FTIR spectroscopy. Results indicate that 25% morin was encapsulated on pSC-3 at pH 7.3 and phase solubility study suggested 10 fold increase in water solubility of morin while 1:1 stoichiometric ratio was confirmed by Job's plot. Binding constant for inclusion complex was found to be 6.67 × 10⁴ M⁻¹ calculated by using the Benesi–Hildebrand equation. In order to study the detailed structural properties of the complex, molecular mechanism approach was applied to calculate different energies associated with complex.

KEYWORDS:

• Inclusion complex
• molecular mechanism
• Morin
• p-sulphonatocalix[4]arene
• solubility
• stoichiometry

Acknowledgements

We are highly thankful to National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro for the financial support.