Rapid Production of Chicken Egg Yolk Antibodies Against Multidrug Resistance-Associated Protein 1 (MRP-1)

Abstract

Resistance of cancer cells to a drug is usually accompanied by resistance to other drugs with different structures and cellular targets. This is called multidrug resistance (MDR). There have been many studies to develop anticancer agents and to identify new tumor markers. Multidrug resistance-associated protein 1 (MRP1), a 190 kDa protein found in a small-cell lung carcinoma line (H69AR), can confer cellular resistance to anthracyclines, vinca alkaloids, and epipodophyllotoxins and is considered to be primarily a multispecific organic anion transporter. A recent study showing the role of MRP1 in endobiotic excretion from cells has led to a suggestion that MRP1 also may have a physiological role in cells. Generation of antibodies in mammals is invasive. In addition, antibody production in mice requires sacrifice of the animals. Production of polyclonal antibodies against a mammalian protein in chicken and isolation of it from the egg yolk seems advantageous since the method is noninvasive, the animal does not need to be sacrificed, and the antibody titers in the eggs are higher than in mammals. Chickens are phylogenetically distant from mammals. Therefore, production of antibodies against a mammalian protein seems easier to produce in chickens.

Keywords:

• egg yolk antibody (IgY)
• multidrug resistance-associated protein 1 (MRP1)

Acknowledgments

This work was supported by the Research Fund of Akdeniz University.