Current Trends in Enzymatic Determination of Glycerol

This review focuses on the enzymatic glycerol detection methods. Importance of glycerol as biologically active compound, chemical glycerol determination methods, and current trends in enzymatic glycerol determination are reviewed. Application of FAD-dependent glycerol oxidases, NAD-dependent dehydrogenases, lipases and multi-enzymatic systems for glycerol and polyglyceride detection by optical and electrochemical methods are analyzed in detail. The application of PQQ-dependent enzymes is predicted as one of alternative and promising ways for detection of glycerol in complex biological samples. Future trends in application of PQQ-dependent glycerol dehydrogenases are predicted.

Keywords:

• biosensor
• PQQ
• glycerol dehydrogenase
• polypyrrole
• electrochemical detection
• food
• beverage
• wine

Acknowledgments

This work was partially financially supported by Lithuanian State Science and Studies Foundation project number C-03047, and COST action D25.

All authors of Sector of Immunoanalysis and Informatics in the Institute of Immunology.